Bactericidal effect of bacteria isolated from the marine sponges Hymeniacidon perlevis and Halichondria panicea against carbapenem-resistant Acinetobacter baumannii
Rodriguez Jimenez, A.; Breine, A.; Whiteway, C.; Dechamps, E.; George, I.F.; Van der Henst, C. (2024). Bactericidal effect of bacteria isolated from the marine sponges Hymeniacidon perlevis and Halichondria panicea against carbapenem-resistant Acinetobacter baumannii. Letters in Applied Microbiology 77(5): ovae035. https://dx.doi.org/10.1093/lambio/ovae035
In: Letters in Applied Microbiology. Blackwell: Oxford. ISSN 0266-8254; e-ISSN 1472-765X, more
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| Keywords |
Pathogens
Halichondria panicea (Pallas, 1766) [WoRMS]; Hymeniacidon perlevis (Montagu, 1814) [WoRMS]
Marine/Coastal |
| Author keywords |
antimicrobial resistance, marine microbiology, lactic acid bacteria, novel antimicrobials |
| Authors | | Top |
- Rodriguez Jimenez, A., more
- Breine, A.
- Whiteway, C.
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- Dechamps, E., more
- George, I.F., more
- Van der Henst, C., more
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| Abstract |
In this study, we evaluated the antimicrobial activity of bacteria isolated from the marine sponges Hymeniacidon perlevis and Halichondria panicea against seven Acinetobacter baumannii strains, the majority of which were clinically relevant carbapenem-resistant A. baumannii strains. We observed the inhibitory activity of 18 (out of 114) sponge-isolated bacterial strains against all A. baumannii strains using medium-throughput solid agar overlay assays. These inhibitory strains belonged to the genera Lactococcus, Pseudomonas, and Vagococcus. In addition, this antimicrobial activity was validated through a liquid co-cultivation challenge using an inhibitory strain of each genus and a green fluorescent protein-tagged A. baumannii strain. Fluorescence measurements indicated that the growth of A. baumannii was inhibited by the sponge isolates. In addition, the inability of A. baumannii to grow after spreading the co-cultures on solid medium allowed us to characterize the activity of the sponge isolates as bactericidal. In conclusion, this study demonstrates that marine sponges are a reservoir of bacteria that deserves to be tapped for antibiotic discovery against A. baumannii. |
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