Molecular phylogenetic analysis of the microbial diversity of a vent cap sample
Corre, E.; Reysenbach, A.-L.; Prieur, D. (1997). Molecular phylogenetic analysis of the microbial diversity of a vent cap sample, in: Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2): pp. 120-121
In: (1997). Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2)[s.n.][s.l.]. 111-149 pp., more
In: Cahiers de Biologie Marine. Station Biologique de Roscoff: Paris. ISSN 0007-9723; e-ISSN 2262-3094, more
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| Authors | | Top |
- Corre, E.
- Reysenbach, A.-L.
- Prieur, D.
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| Abstract |
The microbial diversity of a hydrothermal site has been analysed using a molecular phylogenetic approach. Classical techniques for studying microbial diversity, such as culture-based enrichment techniques, rely on our ability to grow organisms in culture. However, it is well established that we can only retrieve a very small part of the environmental microbial diversity using this approach since enrichment media is highly selective. Recently, molecular phylogenetic techniques based on the small subunit RNA (16S rRNA) have been developed that circumvent this limitation. We chose to use this rRNA-based technology in conjunction with microscopy to analyse the diversity associated with a deep-sea hydrothermal vent system. The samples were collected from an in situ growth chamber ("vent cap") deployed on vent field during the "Microsmoke" oceanographic cruise in November 1995, "Snake Pit", Mid-atlantic Ridge. The DNA was first extracted and the I6S RRNA genes were amplified by PCR using primers specific for the Archaea and Bacteria. The products were sorted by cloning, sequenced and analysed. On a sample, 87 clones corresponding to the domain Bacteria were analysed. According to their RFLP profiles we determined 47 operational taxonomic unit (OTU). Later one representant of each OTU have been sequenced. The totality of the 16S sequenced clones corresponds to none of the previously described bacterial species. They are affiliated to the E subclass of the class Proteobacteria and the more closely related sequences are sequences retrieved from clones obtained from a micobial mat sample collected at a pacific active hydrothermal system (Moyer et at.. 1995). The clones are also more or less affiliated to microaerophilic sulphur or sulphide-oxydizing bacteria (genus Campylobacter, Arcobacter, Helicobacter, Wolinella, Bacteroides, Thiovulum). |
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