Bio-scaffolds produced from irradiated squid pen and crab chitosan with hydroxyapatite/β-tricalcium phosphate for bone-tissue engineering
Shavandi, A.; Bekhit, A.E.-D.A.; Sun, Z.; Ali, M.A. (2016). Bio-scaffolds produced from irradiated squid pen and crab chitosan with hydroxyapatite/β-tricalcium phosphate for bone-tissue engineering. International Journal of Biological Macromolecules 93(Part B): 1446-1456. https://dx.doi.org/10.1016/j.ijbiomac.2016.04.046
In: International Journal of Biological Macromolecules. ELSEVIER SCIENCE BV: Amsterdam. ISSN 0141-8130; e-ISSN 1879-0003, more
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| Author keywords |
Squid pen chitosan; Hydroxyapatite; Irradiation |
| Authors | | Top |
- Shavandi, A., more
- Bekhit, A.E.-D.A.
- Sun, Z.
- Ali, M.A.
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| Abstract |
In this study, bio-scaffolds have been developed using irradiated chitosan from different sources – squid pen (RS) and crab shell (RC) – with hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) at a chitosan/HA/β-TCP ratio of 50/30/20. The bio-scaffolds were prepared at two different freezing temperature (−20 °C and −80 °C) followed by lyophilisation. To enhance the mechanical properties, the bio-scaffolds were cross-linked using sodium tripolyphosphate (TPP) followed by lyophilisation. The composition and morphology of the bio-scaffolds were characterized using XRD, SEM, TEM and μ-CT. The pore size of the porous scaffolds ranged from 90 to 220 μm and the scaffolds had 70–80% porosity. The scaffolds had a water uptake ratio of more than 10, and a controlled biodegradation in the range of 30–40%. These results suggest that the physical and biological properties of chitosan-based bio-scaffolds can be a promising biomaterial for bone-tissue regeneration. |
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