Bacteriophages: Methods and protocols - Volume III
Clokie, M.R.J.; Kropinski, A.M.; Lavigne, R. (Ed.) (2018). Bacteriophages: Methods and protocols - Volume III. Methods in Molecular Biology, 1681. Springer Science+Business Media: New York. ISBN 978-1-4939-7343-9. 316 pp. https://dx.doi.org/10.1007/978-1-4939-7343-9
Part of: Methods in Molecular Biology. Humana Press. ISSN 1064-3745, more
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| Authors | | Top |
- Clokie, M.R.J., editor
- Kropinski, A.M., editor
- Lavigne, R., editor, more
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| Abstract |
This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. |
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