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Immune receptor- and transcription factor-stimulatory activity of bacteria and endotoxin in sea spray aerosols on human cells
Citable as data publication
Li, Y.; Schütte, W.; Dekeukeleire, M.; Janssen, C.; Boon, N.; Asselman, J.; Lebeer, S.; Spacova, I.; De Rijcke, M.; Flanders Marine Institute (VLIZ); University of Antwerp; Ghent University: Belgium; (2024): Immune receptor- and transcription factor-stimulatory activity of bacteria and endotoxin in sea spray aerosols on human cells. Marine Data Archive. https://doi.org/10.14284/658
Contact:
Li, Yunmeng Availability:
 This dataset is licensed under a Creative Commons Attribution 4.0 International License.Description
Total bacterial count and endotoxin concentration in sea spray aerosols, and their immunostimulatory activity on TLR4 in HEK-Blue hTLR4 cells, TLR2/6 in HEK-Blue hTLR2/6 cells, as well as NF-κB and IRF in THP1-Dual monocytes. Scope Themes: Biology Keywords: Marine/Coastal, Endotoxin, Human cells, Immunostimulatory activity, Sea spray aerosols, ANE, Belgium, Oostende Harbour, Bacteria Geographical coverage ANE, Belgium, Oostende Harbour [Marine Regions] Temporal coverage
20 March 2023 - 13 April 2023 Taxonomic coverage
Bacteria [WoRMS]
Parameters
Endotoxin Methodology
IRF activation Methodology NF-κB activation Methodology Sodium (Na) Methodology TLR2/6 activation Methodology TLR4 activation Methodology Total bacterial count Methodology Endotoxin: PyroGene Recombinant Factor C (rFC) assay IRF activation : THP1-Dual monocytes NF-κB activation : THP1-Dual monocytes Sodium (Na): Inductively Coupled Plasma Optical Emission spectroscopy TLR2/6 activation: HEK-Blue hTLR2/6 cells TLR4 activation : HEK-Blue hTLR4 cells Total bacterial count: Flow cytometry Contributors
Vlaams Instituut voor de Zee (VLIZ), more, data creator
University of Antwerp; Department of Bioscience Engineering; Laboratory of Applied Microbiology and Biotechnology (ENdEMIC), more, data creator
Ghent University; Blue Growth Research Lab, more, data creator
Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology (CMET), more, data creator
Publication
Based on this dataset
Li, Y. et al. (2024). The immunostimulatory activity of sea spray aerosols: bacteria and endotoxins activate TLR4, TLR2/6, NF-κB and IRF in human cells. Sci. Total Environ. 927: 171969. https://dx.doi.org/10.1016/j.scitotenv.2024.171969, more
Dataset status: Completed
Data type: Data
Data origin: Research: experiment
Metadatarecord created: 2024-03-07
Information last updated: 2024-03-11
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