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Immunostimulatory effects of marine bacteria and bacterial endotoxin in sea spray aerosols
Citation
Schütte, W., Li, Y., De Rijcke, M. (2023): Immunostimulatory effects of marine bacteria and bacterial endotoxin in sea spray aerosols. Flanders Marine Institute (VLIZ). https://marineinfo.org/id/dataset/8344
Contact:
De Rijcke, Maarten ;
Availability: The data can be made available upon request, please use contact information.
Description
Bacterial counts and endotoxin concentrations of aerosol samples collected at the beach of Ostend, Belgium, and generated in the laboratory with a marine aerosol referenc tank (MART). Data regarding gene induction in human reporter cell lines (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual) after stimulation with these collected aerosols. moreThe data was used to examine levels of bacterial endotoxins in SSA and their effect on cell-signalling pathways of the human innate immune system. Human reporter cell lines (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6 and THP1-Dual) were exposed to aerosol samples collected at the beach of Ostend (Belgium) and generated with a Marine Aerosol Reference Tank (MART) in the laboratory. Bacteria cell count s in aerosol samples were measured with flow cytometry and endotoxins were quantified using the rFC assay. Scope Keywords: Immunology, Marine ecology, Sea spray, Sea spray aerosols, ANE, Belgium, Oostende Geographical coverage ANE, Belgium, Oostende [Marine Regions] Temporal coverage
3 March 2023 - 26 May 2023 Parameters
Count of bacteria Methodology
Endotocin concentration Methodology Gene induction measured at optical density 405nm Methodology Count of bacteria: Flow cytometry Endotocin concentration: rF C assay rF C assay Gene induction measured at optical density 405nm: Reporter cell lines assay (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual cells) Reporter cell lines assay (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual cells) Contributors
Dataset status: Completed
Data type: Data
Data origin: Research: experiment
Metadatarecord created: 2023-06-02
Information last updated: 2023-06-02
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